SARS-CoV-2 (COVID-19) Spike S2 Antibody

• Catalog Number: orb1238694
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) Spike S2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Virus
• Isotype: IgG
• Immunogen: Anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (orb1238694) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein. The immunogen is located within 1130-1180 amino acids of SARS-CoV-2 (COVID-19) Spike protein.
• Concentration: 1 mg/mL
• Dilution range: WB: 1 μg/mL; IHC: 0.1 μg/mL; IF: 10 μg/mL.Antibody validated: Immunohistochemistry and Immunofluorescence in human samples. SARS-CoV-2 (COVID-19) Spike antibody can be used for the detection of SARS-CoV-2 (COVID-19) Spike protein in ELISA. It will detect 4 ng of free peptide at 1 μg/mL. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S
• UniProt ID: P0DTC2
• NCBI: QHD43416
• Storage: SARS-CoV-2°COVID-19) Spike antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) Spike Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: SARS-CoV-2 (COVID-19) Spike Antibody: Severe acute
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Spike S2 in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (orb1238694, 0.1 µg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 spike protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.

ELISA Validation with SARS-CoV-2 (COVID-19) Spike S2 ECD Recombinant Protein. Antibodies: SARS-CoV-2 (COVID-19) Spike S2 antibody, orb1238694. A direct ELISA was performed using SARS-CoV-2 (COVID-19) Spike S2 ECD recombinant protein (orb1231609) as coating antigen and the anti-SARS-CoV-2 (COVID-19) Spike S2 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 8 ng/mL to 1000 ng/mL.

Western Blot Validation with SARS-CoV-2 (COVID-19) Spike S2 ECD Recombinant Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Spike S2 ECD recombinant protein, orb1231609. Antibodies: SARS-CoV-2 (COVID-19) Spike S2, orb1238694, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL and Lane 2: 2 µg/mL.

Overexpression Validation in Spike Transfected 293 Cells. Loading: 10 µg per lane of Spike transfected 293 cell lysate. Antibodies: SARS-CoV-2 (COVID-19) Spike, orb1238694, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL and Lane 2: 2 µg/mL.