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Catalog Number | orb1231606 |
---|---|
Category | Proteins |
Description | SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein |
Tested applications | ELISA, WB |
Tag | His tag at the C-terminus |
Dilution range | This recombinant protein can be used for SDS-PAGE. |
Form/Appearance | Lyophilized |
Purity | > 90% by SDS-PAGE. |
MW | 76 kDa |
Target | S |
UniProt ID | P0DTC2 |
Protein Sequence | Val11-Arg683 |
Source | HEK293 cells |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Recombinant 2019-nCoV Spike S1-His at 2μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 1.5-36.5 ng/mL. |
NCBI | YP_009724390.1 |
Storage | Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. |
Buffer/Preservatives | PBS, pH 7.4 |
Alternative names | S1 protein, Spike glycoprotein Subunit1, S glycopr Read more... |
Note | For research use only |
Application notes | This recombinant protein can be used for SDS-PAGE. |
Expiration Date | 6 months from date of receipt. |
Immobilized SARS-CoV-2 (COVID-19) spike S1 recombinant protein at 2μg/mL (100 μL/well) can bind recombinant human ACE2 with a linear range of 1.5-36.5 ng/mL.
SARS-CoV-2 (COVID-19) spike S1 recombinant protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 110-130 kDa.
ELISA, SDS-PAGE, WB | |
> 90% as determined by Bis-Tris PAGE | |
120 kD | |
HEK293 cells |
ELISA, WB | |
< 95% | |
42.9 kD | |
HEK293 cells |
ELISA, WB | |
< 95% | |
31.8 kD | |
HEK293 cells |
ELISA, WB | |
< 95% | |
24.7 kD | |
HEK293 cells |
ELISA, WB | |
< 95% | |
76.2 kD | |
HEK293 cells |
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