SARS-CoV-2 (COVID-19) Delta Variant Spike S1 (His-Avi Tag) Recombinant Protein

• Catalog Number: orb1228206
• Category: Proteins
• Description: SARS-CoV-2 (COVID-19) Delta Variant Spike S1 (His-Avi Tag) Recombinant Protein
• Tested applications: ELISA, SDS-PAGE, WB
• Tag: His-Avi tag at C-terminus
• Form/Appearance: Liquid
• Purity: > 90% as determined by Bis-Tris PAGE
• MW: 120 kD
• Target: S
• UniProt ID: P0DTC1
• Protein Sequence: Val 16 - Arg 685 (T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R)-His-Avi
• Source: HEK293 cells
• NCBI: QHD43416.1
• Storage: Store in working aliquots at -70˚°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Buffer/Preservatives: PBS (pH 7.4).
• Alternative names: S protein RBD, Spike glycoprotein Receptor-binding
• Note: For research use only
• Application notes: This recombinant protein can be used for WB and ELISA. For research use only.
• Expiration Date: 6 months from date of receipt.

ELISA Binding Assay of ACE2 and SARS-CoV-2 (COVID-19) Delta Variant Spike S1 Recombinant Protein. Coating Antigen: SARS-CoV-2 Delta variant (B.1.671.2) spike S1 protein, 1 μg/mL, incubated at 4 °C overnight. Detection Antibodies: ACE2 recombinant protein, orb1231604, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-human HRP at 1:10, 000, incubate at RT for 1 hr.

SDS-PAGE Validation of SARS-CoV-2 (COVID-19) Delta Variant Spike S1 Recombinant Protein. Loading: 1 μg SARS-CoV-2 (COVID-19) Delta variant Spike S1 recombinant protein, orb1228206. Observed: 120kD

ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 °C overnight. Detection Antibodies: SARS-CoV-2 Delta variant spike 156-157EFdel antibody, orb1239050, and spike 156-157EF antibody, orb1239051, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20, 000, incubate at RT for 1 hr. SARS-CoV-2 Delta Variant Spike S1 protein (orb1228206), but not WT spike S1 protein (orb1231589), was specifically detected by Delta Variant spike antibody (orb1239050), while the corresponding WT antibody (orb1239051) detected spike S1 protein of WT, but not delta variant.

ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 °C overnight. Detection Antibodies: SARS-CoV-2 Delta variant spike P681R antibodies, dilution: 200 and 1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5, 000, incubate at RT for 1 hr. SARS-CoV-2 Delta Variant Spike S1 protein (orb1228206), but not WT spike S1 protein (orb1231589), was specifically detected by Delta Variant spike antibodies.

WB Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Delta Variant Spike Antibodies. Loading: 20 ng of SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2). Detection Antibodies: SARS-CoV-2 Delta variant spike antibody, orb1239050, 1 μg/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20, 000, incubate at RT for 1 hr. SARS-CoV-2 Delta Variant S1 protein (orb1228206), but not WT spike S1 protein (orb1231589), was specifically detected by Delta Variant spike antibody (orb1239050).

ELISA Validation of Delta Variant Spike S1 Protein with SARS-CoV-2 Spike S1 Antibodies. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 °C overnight. Detection Antibodies: SARS-CoV-2 Spike S1 antibody, orb1239995, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20, 000, incubate at RT for 1 hr. Antibody orb1239995 does not distinguish between WT and Delta.