SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein

• Catalog Number: orb1231589
• Category: Proteins
• Description: SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein
• Tested applications: ELISA, WB
• Tag: His tag at C-terminus
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Purity: < 95%
• MW: 76.2 kD
• Protein Sequence: Val16-Arg685
• Source: HEK293 cells
• Storage: The protein is stable in a liquid state at -70°C for 12 months. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS
• Alternative names: SARS-CoV-2 S, coronavirus spike Protein, cov spike
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

ELISA Validation of SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein, Coating Antigen: SARS-CoV-2 (COVID-19) Spike S1 recombinant protein (orb1231589) from 0.3 ng to 1000 ng. Capture Antibody: SARS-CoV-2 (COVID-19) Spike S1 antibody, orb1239981 (1 μg/mL). Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution.

SDS-PAGE Validation with SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein. Loading: 1 μg SARS-CoV-2 (COVID-19) Spike S1 recombinant protein, orb1231589. Observed: 125kD

Binding Assay of ACE2 and SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein. Coating: SARS-CoV-2 (COVID-19) Spike S1 recombinant protein (orb1231589) from 3 ng to 1000 ng. Capture: ACE2 recombinant protein, orb1231604 (0.5 μg/mL). Secondary: Goat anti-human IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation with SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Spike S1 recombinant protein, orb1231589. Antibodies: SARS-CoV-2 (COVID-19) Spike S1, orb1239981, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 μg/mL and Lane 2: 2 μg/mL.