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Catalog Number | orb1231588 |
---|---|
Category | Proteins |
Description | SARS-CoV-2 (COVID-19) Spike RBD Recombinant Protein |
Tested applications | ELISA, WB |
Tag | His tag at C-terminus |
Form/Appearance | Liquid |
Purity | < 95% |
MW | 24.7 kD |
Protein Sequence | Arg319-Phe541 |
Source | HEK293 cells |
Storage | The protein is stable in a liquid state at -70°C for 12 months. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS |
Alternative names | SARS-CoV-2 S, coronavirus spike Protein, cov spike Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Binding Assay of ACE2 and SARS-CoV-2 (COVID-19) Spike RBD Recombinant Protein, Coating: SARS-CoV-2 (COVID-19) Spike RBD recombinant protein (orb1231588) from 0.3 ng to 1000 ng. Capture: ACE2 recombinant protein, orb1231604 (0.5 μg/mL). Secondary: Goat anti-human IgG HRP conjugate at 1:10000 dilution.
SDS-PAGE Validation with SARS-CoV-2 (COVID-19) Spike RBD Recombinant Protein. Loading: 1 μg SARS-CoV-2 (COVID-19) Spike RBD recombinant protein, orb1231588. Observed: 35kD
Western Blot Validation with SARS-CoV-2 (COVID-19) Spike RBD Recombinant Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) Spike RBD recombinant protein, orb1231588. Antibodies: SARS-CoV-2 (COVID-19) Spike RBD, orb1239994, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 μg/mL and Lane 2: 2 μg/mL.
ELISA Validation of SARS-CoV-2 (COVID-19) Spike RBD Recombinant Protein. Coating Antigen: SARS-CoV-2 (COVID-19) Spike RBD recombinant protein (orb1231588) from 0.3 ng to 1000 ng. Capture Antibody: SARS-CoV-2 (COVID-19) Spike RBD antibody, orb1239994 (1 μg/mL). Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution.
ELISA, SDS-PAGE, WB | |
> 90% as determined by Bis-Tris PAGE | |
120 kD | |
HEK293 cells |
ELISA, SDS-PAGE, WB | |
> 95% as determined by Bis-Tris PAGE | |
35 kD | |
HEK293 cells |
ELISA, WB | |
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLCEndotoxin level is less than 1EU per ug by the LAL method. | |
HEK293 cells |
ELISA, WB | |
< 95% | |
42.9 kD | |
HEK293 cells |
ELISA, WB | |
< 95% | |
31.8 kD | |
HEK293 cells |