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Catalog Number | orb1671226 |
---|---|
Category | Proteins |
Description | SARS-CoV-2 (Covid-19) spike receptor binding domain Recombinant Protein |
Tested applications | CLIA, ELISA, LF, WB |
Concentration | batch dependent |
Form/Appearance | liquid |
Purity | > 95% pure by SDS-PAGE. Ligand affinity purified. |
MW | This recombinant protein contains Covid-19 spike protein receptor binding domain (S-RBD, Arg319-Phe541) with a MW of 29.17 kDa. It is recognized by an anti-S-RBD antibody in ELISA tests. |
Source | Insect cell |
Storage | Upon receipt, quick spin before open vial, aliquot and store at ≤ –20°C. Avoid multiple freeze/thaw cycles |
Buffer/Preservatives | PBS, pH 7.3 containing 0.1% ProClin300. |
Note | For research use only |
Application notes | Suitable for use in ELISA, CLIA, Lateral Flow, Western Blot, and other immune tests |
Expiration Date | 6 months from date of receipt. |
SDS-Page Image
ELISA, SDS-PAGE, WB | |
> 95% as determined by Bis-Tris PAGE | |
35 kD | |
HEK293 cells |
ELISA, SDS-PAGE, WB | |
> 90% as determined by Bis-Tris PAGE | |
120 kD | |
HEK293 cells |
ELISA, WB | |
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLCEndotoxin level is less than 1EU per ug by the LAL method. | |
HEK293 cells |
ELISA, WB | |
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC | |
Predicted: 51.7kD Observed: 60-62kD due to glycosylation | |
HEK293 cells |
ELISA, WB | |
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC | |
Predicted: 27kD Observed: 36-40kD due to glycosylation | |
HEK293 cells |
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