SARS-CoV-2 (COVID-19) UK variant (B.1.1.7) S RBD (N501Y) Recombinant Protein

• Catalog Number: orb1231548
• Category: Proteins
• Description: SARS-CoV-2 (COVID-19) UK variant (B.1.1.7) S RBD (N501Y) Recombinant Protein
• Tested applications: ELISA, WB
• Tag: His-Avi
• Dilution range: SDS-PAGE
• Form/Appearance: Lyophilized
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLCEndotoxin level is less than 1EU per ug by the LAL method.
• Protein Sequence: Arg319 - Phe541 (N501Y)
• Source: HEK293 cells
• NCBI: QHD43416.1
• Storage: Store in working aliquots at -20°C. Avoid freeze/thaw cycles.
• Buffer/Preservatives: 20mM PB (pH 7.4). 5 % trehalose is added as protectant before lyophilization.
• Alternative names: S protein RBD, Spike glycoprotein Receptor-binding
• Note: For research use only
• Application notes: SDS-PAGE
• Expiration Date: 6 months from date of receipt.

Immobilized SARS-CoV S protein RBD (N501Y), His Tag at 0.5 ug/ml (100ug/Well) on the plate. Dose response curve for Human ACE2, Fc Tag with the EC50 of 12.7 ng/ml determined by ELISA.

The purity of SARS-COV-2 S protein RBD (N501Y) is greater than 95% as determiend by SEC-HPLC.

Human ACE2 captured in protein A chip, can bind SARS-CoV S protein RBD (N501Y), His Tag with an affinity constant of 1.74nM as determined in SPR assap (Biacore T200).

SARS-COV2 S protein RBD (N501Y) on Tris-Bis PAGE under reduced condition. The purity os great than 95%.