SARS-CoV-2 (COVID-19) Delta Variant Spike RBD (His-Avi Tag) Recombinant Protein

• Catalog Number: orb1228207
• Category: Proteins
• Description: SARS-CoV-2 (COVID-19) Delta Variant Spike RBD (His-Avi Tag) Recombinant Protein
• Tested applications: ELISA, SDS-PAGE, WB
• Tag: His-Avi tag at C-terminus
• Form/Appearance: Liquid
• Purity: > 95% as determined by Bis-Tris PAGE
• MW: 35 kD
• Target: S
• UniProt ID: P0DTC1
• Protein Sequence: Arg319-Phe541 (T478K, L452R)-His-Avi
• Source: HEK293 cells
• NCBI: QHD43416.1
• Storage: Store in working aliquots at -70˚°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Buffer/Preservatives: PBS (pH 7.4).
• Alternative names: S protein RBD, Spike glycoprotein Receptor-binding
• Note: For research use only
• Application notes: This recombinant protein can be used for WB and ELISA. For research use only.
• Expiration Date: 6 months from date of receipt.

ELISA Binding Assay of ACE2 and SARS-CoV-2 (COVID-19) Delta Variant Spike RBD Recombinant Protein, Coating Antigen: SARS-CoV-2 Delta variant (B.1.671.2) spike RBD protein, 1 μg/mL, incubated at 4 °C overnight. Detection Antibodies: ACE2 recombinant protein, orb1231604, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-human HRP at 1:10, 000, incubate at RT for 1 hr.

SDS-PAGE Validation of SARS-CoV-2 (COVID-19) Delta Variant Spike RBD Recombinant Protein. Loading: 1 μg SARS-CoV-2 (COVID-19) Delta variant Spike RBD recombinant protein, orb1228207. Observed: 35kD

ELISA Validation of Delta Variant Spike RBD Protein with SARS-CoV-2 Delta Variant Spike L452R Antibodies. Coating Antigen: SARS-CoV-2 spike RBD proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 °C overnight. Detection Antibodies: SARS-CoV-2 Delta variant spike L452R antibody, orb1239036, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20, 000, incubate at RT for 1 hr. SARS-CoV-2 Delta Variant RBD protein (orb1228207), but not WT spike RBD protein (orb1231588), was specifically detected by Delta Variant spike L452R antibody (orb1239036).

WB Validation of Delta Variant Spike RBD Protein with SARS-CoV-2 Delta Variant Spike L452R Antibodies. Loading: 20 ng of SARS-CoV-2 spike RBD proteins, including WT and Delta variant (B.1.671.2). Detection Antibodies: SARS-CoV-2 Delta variant spike L452R antibody, orb1239036, 1 μg/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20, 000, incubate at RT for 1 hr. SARS-CoV-2 Delta Variant RBD protein (orb1228207), but not WT spike RBD protein (orb1231588), was specifically detected by Delta Variant spike L452R antibody (orb1239036).

ELISA Validation of Delta Variant Spike RBD Protein with SARS-CoV-2 Spike RBD Antibodies. Coating Antigen: SARS-CoV-2 spike RBD proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 °C overnight. Detection Antibodies: SARS-CoV-2 Spike RBD antibody, orb1239994, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20, 000, incubate at RT for 1 hr. Antibody orb1239994 does not distinguish between WT and Delta.

WB Validation of Delta Variant Spike RBD Protein with SARS-CoV-2 Spike RBD Antibodies. Loading: 20 ng of SARS-CoV-2 spike RBD proteins, including WT and Delta variant (B.1.671.2). Detection Antibodies: SARS-CoV-2 Spike RBD antibody, orb1239994, 1 μg/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20, 000, incubate at RT for 1 hr.