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Catalog Number | orb640164 |
---|---|
Category | Proteins |
Description | NSP8, forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers. To ultimately combat the emerging COVID-19 pandemic, it is desired to develop an effective and safe vaccine against this highly contagious disease caused by the SARS-CoV-2 coronavirus. By investigating the entire proteome of SARS-CoV-2, six proteins, including the S protein and five non-structural proteins (nsp3, 3CL-pro, and nsp8-10) were predicted to be adhesins, which are crucial to the viral adhering and host invasion. The S, nsp3, and nsp8 proteins were also predicted by Vaxign-ML to induce high protective antigenicity. |
Reactivity | Virus |
Tag | N-10×His |
Form/Appearance | Liquid |
Purity | 95% |
Conjugation | Unconjugated |
MW | 23.9 kDa |
Target | NSP8 |
Protein Sequence | YP_009725304.1 |
Source | SARS-CoV-2 NSP8, His Tag (orb640164) is expressed from E. coli cells. It contains AA Ala 1 - Gln 198 (Accession # YP_009725304.1). |
Expression System | E. coli |
Expression Region | Ala 1 - Gln 198 |
Endotoxins | 1.0 EU per μg |
NCBI | YP_009725304.1 |
Storage | -70℃ |
Buffer/Preservatives | 20 mM Tris, 250 mM NaCl, pH8.5 |
Alternative names | NSP8,nsp8,Non-structural protein 8 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 23.9 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE). |
Expiration Date | 6 months from date of receipt. |
WB | |
> 95% as determined by SDS-PAGE. | |
22-25 kDa | |
E. coli |
GSH-Agarose (0.95) | |
Mono-Isotopic Mass: 48, 673.74 daltons Average Mass: 48, 705.25 daltons | |
bacteria |
Amylose Resin (0.95) | |
Mono-Isotopic Mass: 66, 078.48 daltons Average Mass: 66, 120.00 daltons | |
bacteria |
> 85% as determined by SDS-PAGE quantitative densitometry by Coomassie Blue Staining. | |
24.18kDa | |
E. coli |
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