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Catalog Number | orb1231057 |
---|---|
Category | Proteins |
Description | SARS-CoV-2 (COVID-19) NSP8 Recombinant Protein |
Target | ORF1ab |
Tag | N-Term GST Uncleaved |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | 50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35 |
Purity | GSH-Agarose (0.95) |
Protein Sequence | Native Sequence:MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAIASEFSSLPSYAAFATAQEAYEQAVANGDSEVVLKKLKKSLNVAKSEFDRDAAMQRKLEKMADQAMTQMYKQARSEDKRAKVTSAMQTMLFTMLRKLDNDALNNIINNARDGCVPLNIIPLTTAAKLMVVIPDYNTYKNTCDGTTFTYASALWEIQQVVDADSKIVQLSEISMDNSPNLAWPLIVTALRANSAVKLQAmino acids A1 – Q198 (end).Residue A232 of the fusion protein is equivalent to A1 of the native enzyme.The GST tag is located at residues 1 – 220.Protease Cleavage:PreScission (LEVLFQGP) residues 221 - 228 |
UniProt ID | P0DTC1 |
MW | Mono-Isotopic Mass: 48, 673.74 daltons Average Mass: 48, 705.25 daltons |
Source | bacteria |
Storage | Store at -70 °C. Avoid repeated freeze-thaw cycles. |
Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
Alternative names | SARS-CoV2-NSP8, SARS-CoV-2 NSP8, NSP8 SARS-CoV-2 |
Note | For research use only |
NCBI | QHD43415.1 |
WB | |
> 95% as determined by SDS-PAGE. | |
22-25 kDa | |
E. coli |
Amylose Resin (0.95) | |
Mono-Isotopic Mass: 66, 078.48 daltons Average Mass: 66, 120.00 daltons | |
bacteria |
> 85% as determined by SDS-PAGE quantitative densitometry by Coomassie Blue Staining. | |
24.18kDa | |
E. coli |
≥ 90% as determined by SDS-PAGE | |
Recombinant SARS-CoV-2 NSP8 was expressed from E.coli expression system and the target gene encoding Ala1-Gln198 is expressed with N-His Tag |