S100B (Astrocyte and Melanoma Marker) Antibody

• Catalog Number: orb1410203
• Category: Antibodies
• Description: Mouse monoclonal antibody to S100B (Astrocyte and Melanoma Marker)
• Target: S100B
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Immunogen: Recombinant fragment (around aa3-92) of human S100B protein (exact sequence is proprietary)
• UniProt ID: P04271
• MW: 10-12kDa
• Tested applications: IHC
• Dilution range: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Application notes: Positive Control: Human brain or melanoma tissue
• Clone Number: S100B/4149
• Expression System: HEK293 cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NEF; Protein S100-B; S-100 protein beta chain; S10
• Note: For research use only
• Entrez: 6285
• Expiration Date: 12 months from date of receipt.