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S100B Antibody

Catalog Number: orb1930681

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1930681
CategoryAntibodies
DescriptionAffinity Purified Rabbit Polyclonal Antibody (Pab)
Species/HostRabbit
ClonalityPolyclonal
Clone NumberRB22934
Tested applicationsFC, IHC-P, WB
Predicted ReactivityRabbit
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
Antibody TypePrimary Antibody
Dilution rangeWB: 1:2000, WB: 1:1000, WB: 1:2000, IHC-P-Leica: 1:500, IHC-P-Leica: 1:500, FC: 1:10~50, IHC: 1:1000
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
ConjugationUnconjugated
MW10713 Da
TargetThis S100B antibody is generated from rabbits immunized with S100B recombinant protein.
UniProt IDP04271
NCBINP_006263.1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesProtein S100-B, S-100 protein beta chain, S-100 pr
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NoteFor research use only
Expiration Date12 months from date of receipt.
S100B Antibody

Immunohistochemical analysis of paraffin-embedded Human brain section using Pink1. Diluted at 1:1000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

S100B Antibody

S100B Antibody flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

S100B Antibody

Immunohistochemical analysis of paraffin-embedded Human breast tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

S100B Antibody

Immunohistochemical analysis of paraffin-embedded Human melanoma tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

S100B Antibody

Anti-S100B Antibody at 1:2000 dilution + Human brain whole tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 11 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

S100B Antibody

Western blot analysis of lysates from A431 cell line, mouse brain, rat brain tissue (from left to right), using S100B Antibody. Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.

S100B Antibody

All lanes: Anti-S100B Antibody at 1:2000 dilution. Lane 1: Human brain lysate. Lane 2: C2C12 whole cell lysate. Lane 3: Rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 11 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

  • S100B Rabbit Polyclonal Antibody [orb544589]

    IF,  IHC-Fr,  IHC-P,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 50 μl, 100 μl
  • S100B Rabbit Polyclonal Antibody [orb11345]

    IF,  IHC-Fr,  IHC-P,  WB

    Bovine, Gallus, Rabbit

    Human

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl
  • S100B Mouse Monoclonal Antibody [orb500644]

    IF,  IHC-Fr,  IHC-P,  WB

    Mouse, Rat

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    200 μl, 50 μl, 100 μl
  • S100B Antibody [orb750042]

    IHC-P,  WB

    Human

    Rabbit

    Recombinant

    Unconjugated

    20 μg
  • S100B Antibody [orb750102]

    IF,  IHC-P

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg