S100B (Astrocyte and Melanoma Marker) Antibody

SKU: orb1410351

Description

Mouse monoclonal antibody to S100B (Astrocyte and Melanoma Marker)

Images & Validation

• Tested Applications: IHC
• Reactivity: Bovine, Human, Mouse, Rat

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: S100B/4153
• Immunogen: Recombinant full-length human S100B protein
• Target: S100B
• Source/Expression System: Bioreactor
• Molecular Weight: 10-12kDa
• Purification: Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Disclaimer: For research use only

Alternative Names

NEF; S-100 protein beta chain; S100 calcium binding protein beta (neural); S100 calcium-binding protein B

Analysis of Protein Array containing more than 19000 full-length human proteins using S100B-Monospecific Mouse Monoclonal Antibody (S100B/4153). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human brain stained with S100B Mouse Monoclonal Antibody (S100B/4153) at 2 ug/ml. HIER: Tris/EDTA, pH9.0, 45 min. 2°C: HRP-polymer, 30 min. DAB, 5 min.

Formalin-fixed, paraffin-embedded human brain stained with S100B Mouse Monoclonal Antibody (S100B/4153) at 2 ug/ml. Inset: PBS instead of primary antibody, secondary negative control.

SDS-PAGE Analysis S100B Mouse Monoclonal Antibody (S100B/4153). Confirmation of Integrity and Purity of Antibody.

Quick Database Links

Gene Symbol

S100B

UniProt

P04271