S100B Antibody

SKU: orb1252144

Description

S100B Antibody

Research Area

Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 µg/mLWestern blot: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.25-0.5 µg/mL for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the S100 beta antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone No.: S100B/1012
• Immunogen: Recombinant full-length human protein was used as the immunogen for the S100 beta antibody.
• Target: S100B
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B

Analysis of HuProt(TM) microarray containing more than 19000 full-length human proteins using S100 beta antibody (clone S100B/1012). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

(Left) Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100 beta antibody (green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Right) Negative control.

IHC testing of FFPE human schwanoma with S100 beta antibody (clone S100B/1012). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human melanoma with S100 beta antibody (clone S100B/1012). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE Analysis of Purified, BSA-Free S100 beta Antibody (clone S100B/1012). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

S100B

UniProt

P04271