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S100B Antibody

Catalog Number: orb750102

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb750102
CategoryAntibodies
DescriptionS100 belongs to the family of calcium binding proteins. S100 alpha and S100 beta proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S100A and S100B) but not on the alpha-chain of S100 (i.e. in S100A and S100A0). This antibody can be used to localize S100A and S100B in various tissue sections. S100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S100 protein.
Species/HostMouse
ClonalityMonoclonal
Clone NumberS100B/1012
Tested applicationsIF, IHC-P
ReactivityHuman
IsotypeMouse IgG2a, kappa
ImmunogenRecombinant full-length human protein was used as the immunogen for the S100 beta antibody.
Antibody TypePrimary Antibody
Dilution rangeImmunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis S100 beta antibody is available for research use only.
UniProt IDP04271
StorageStore the S100 beta antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the S100 beta antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
S100B Antibody

Protein array validation of the S100 beta antibody: Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using S100 beta antibody (clone S100B/1012). These results demonstrate the foremost specificity of the S100B/1012 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

S100B Antibody

(Left) Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100 beta antibody (green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue). (Right) Negative control.

S100B Antibody

IHC testing of FFPE human schwanoma with S100 beta antibody (clone S100B/1012). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

S100B Antibody

IHC testing of FFPE human melanoma with S100 beta antibody (clone S100B/1012). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

S100B Antibody

SDS-PAGE Analysis of Purified, BSA-Free S100 beta Antibody (clone S100B/1012). Confirmation of Integrity and Purity of the Antibody.

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