S100B Antibody

• Catalog Number: orb402954
• Category: Antibodies
• Description: S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. In the developing CNS it acts as a neurotrophic factor and neuronal survival protein. In the adult organism it is usually elevated due to nervous system damage, which makes it a potential clinical marker. [Wiki]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PS1B1-1
• Tested applications: IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Recombinant full-length human S100B protein was used as the immunogen for the S100B antibody.
• Dilution range: Immunofluorescence: 1-2ug/ml,Immunohistology (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This S100B antibody is available for research use only.
• UniProt ID: P04271
• Storage: Store the S100B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the S100B antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human melanoma using S100B antibody

Immunohistochemical staining of Human schwanoma using S100B antibody

Immunofluorescence analysis of A2058 cells using S100B antibody