S100B Antibody

SKU: orb1252188

Description

S100B Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 µg/mLWB: 0.5-1 µg/mLIHC (FFPE): 0.5-1 µg/mL for 30 minutes at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the S100B antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone No.: 4C4.9
• Immunogen: Purified bovine brain S100 protein was used as the immunogen for this S100B antibody.
• Target: S100B
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B

Western blot testing of human samples using S100B antibody (clone 4C4.9). Predicted molecular weight: 12-15 kDa.

IHC testing of human melanoma stained with S100B antibody + AEC chromogen (clone 4C4.9). No special pretreatment is required for IHC staining of formalin-paraffin tissues.

Immunofluorescence testing of A2058 cells and Alexa Fluor 488 labeled S100B antibody (clone 4C4.9).

Quick Database Links

Gene Symbol

S100B

UniProt

P04271