S100A4 Antibody / FSP1

• Catalog Number: orb2636066
• Category: Antibodies
• Description: S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, a6b4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant fragment (within amino acids 1-101) of human S100A4 protein was used as the immunogen for the S100A4 antibody.
• UniProt ID: P26447
• Tested applications: IHC-P, WB
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml
• Application notes: Optimal dilution of the S100A4 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: S100A4/6799
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HeLa cell lysate using S100A4 antibody (clone S100A4/6799). Predicted molecular weight ~12 kDa.

IHC staining of FFPE human tonsil tissue with S100A4 antibody (clone S100A4/6799). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free S100A4 antibody (clone S100A4/6799) as confirmation of integrity and purity.