S100A4 Antibody / FSP1

SKU: orb606840

Description

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human, Mouse
• Application Notes:
  The optimal dilution of the recombinant S100A4 antibody for each application should be determined by the researcher. 1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: S100A4/2750R
• Immunogen: A portion of amino acids 1-200 from the human protein was used as the immunogen for this recombinant S100A4 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

SDS-PAGE analysis of purified, BSA-free recombinant S100A4 antibody as confirmation of integrity and purity.

Western blot testing of human HeLa cell lysate with recombinant S100A4 antibody. Predicted molecular weight ~12 kDa.

IHC testing of FFPE human placenta with recombinant S100A4 antibody. Required HIER: steam sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min and allow to cool before testing.

Immunofluorescent staining of permeabilized human A549 cells with recombinant S100A4 antibody (green, clone S100A4/2750R) and Reddot (red, nuclear stain).

FACS testing of permeabilized human A549 cells with recombinant S100A4 antibody (blue, clone S100A4/2750R) and isotype control (red).

Quick Database Links

UniProt

P26447