S100A4 Antibody / FSP1

• Catalog Number: orb606840
• Category: Antibodies
• Description: S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: S100A4/2750R
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG, kappa
• Immunogen: A portion of amino acids 1-200 from the human protein was used as the immunogen for this recombinant S100A4 antibody.
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant S100A4 antibody is available for research use only.
• UniProt ID: P26447
• Storage: Store the recombinant S100A4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The optimal dilution of the recombinant S100A4 antibody for each application should be determined by the researcher. 1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE analysis of purified, BSA-free recombinant S100A4 antibody as confirmation of integrity and purity.

FACS testing of permeabilized human A549 cells with recombinant S100A4 antibody (blue, clone S100A4/2750R) and isotype control (red).

Immunofluorescent staining of permeabilized human A549 cells with recombinant S100A4 antibody (green, clone S100A4/2750R) and Reddot (red, nuclear stain).

IHC testing of FFPE human placenta with recombinant S100A4 antibody. Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human HeLa cell lysate with recombinant S100A4 antibody. Predicted molecular weight ~12 kDa.