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S100A4 Antibody / FSP1

Catalog Number: orb750071

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb750071
CategoryAntibodies
DescriptionS100A4/FSP1 (Fibroblast specific protein 1) belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including p53, p63, non-muscle myosin IIA, a6b4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
ClonalityMonoclonal
Species/HostMouse
IsotypeMouse IgG1, kappa
ConjugationUnconjugated
ReactivityHuman, Mouse
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
PurityProtein G affinity chromatography
ImmunogenHuman recombinant S100A4 protein was used as the immunogen for this S100A4 antibody.
UniProt IDP26447
Tested applicationsFACS, IF, IHC-P, WB
Dilution rangeFlow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application notesThe optimal dilution of the S100A4 antibody for each application should be determined by the researcher.
Antibody TypePrimary Antibody
Clone NumberS100A4/1482
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageStore the S100A4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Hazard InformationThis S100A4 antibody is available for research use only.
NoteFor research use only
S100A4 Antibody / FSP1

Western blot testing of human 1) HeLa, 2) A549 and 3) A431 cell lysate with S100A4 antibody (clone S100A4/1482). Predicted molecular weight ~12 kDa.

S100A4 Antibody / FSP1

IHC testing of FFPE human placenta with S100A4 antibody (clone S100A4/1482). Required HIER: steam sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling.

S100A4 Antibody / FSP1

Western blot testing of human HeLa cell lysate with S100A4 antibody (clone S100A4/1482). Predicted molecular weight ~12 kDa.

S100A4 Antibody / FSP1

Flow cytometry testing of human T98G cells with S100A4 antibody (clone S100A4/1482); Red = isotype control, Blue = S100A4 antibody.

S100A4 Antibody / FSP1

Flow cytometry testing of human A549 cells with S100A4 antibody (clone S100A4/1482); Red = isotype control, Blue = S100A4 antibody.

S100A4 Antibody / FSP1

SDS-PAGE analysis of purified, BSA-free S100A4 antibody (clone S100A4/1482) as confirmation of integrity and purity.

S100A4 Antibody / FSP1

IF/ICC testing of fixed and permeabilized human HeLa cells with FSP1 antibody (clone S100A4/1482, green) and Reddot nuclear stain (red).

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    Monoclonal

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    20 μg
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    FACS,  IF,  IHC-P,  WB

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    FACS,  IF,  IHC-P,  WB

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    FACS,  IF,  IHC-P,  WB

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    FACS,  IF,  IHC-P,  WB

    Human, Mouse

    Mouse

    Recombinant

    Unconjugated

    100 μg