S100A4 Antibody / FSP1

SKU: orb750071

Description

S100A4/FSP1 (Fibroblast specific protein 1) belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including p53, p63, non-muscle myosin IIA, a6b4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human, Mouse
• Application Notes:
  The optimal dilution of the S100A4 antibody for each application should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: S100A4/1482
• Immunogen: Human recombinant S100A4 protein was used as the immunogen for this S100A4 antibody.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Western blot testing of human 1) HeLa, 2) A549 and 3) A431 cell lysate with S100A4 antibody (clone S100A4/1482). Predicted molecular weight ~12 kDa.

IHC testing of FFPE human placenta with S100A4 antibody (clone S100A4/1482). Required HIER: steam sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling.

Western blot testing of human HeLa cell lysate with S100A4 antibody (clone S100A4/1482). Predicted molecular weight ~12 kDa.

Flow cytometry testing of human T98G cells with S100A4 antibody (clone S100A4/1482); Red = isotype control, Blue = S100A4 antibody.

Flow cytometry testing of human A549 cells with S100A4 antibody (clone S100A4/1482); Red = isotype control, Blue = S100A4 antibody.

SDS-PAGE analysis of purified, BSA-free S100A4 antibody (clone S100A4/1482) as confirmation of integrity and purity.

IF/ICC testing of fixed and permeabilized human HeLa cells with FSP1 antibody (clone S100A4/1482, green) and Reddot nuclear stain (red).

Quick Database Links

UniProt

P26447