KIAA0226 Antibody

SKU: orb1239104

Description

KIAA0226 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Rubicon body can be used for detection of Rubicon by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon.The immunogen is located within amino acids 240 - 290 of Rubicon.
• Target: KIAA0226
• Molecular Weight: Predicted: 90, 101, 109 kDa Observed: 91, 99 kDa
• Purification: Rubicon antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rubicon antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Run domain Beclin-1 interacting and cysteine-rich containing protein, KIAA0226

Western blot analysis of Rubicon in 293 cell lysate with Rubicon antibody at 1 µg/ml.

Immunohistochemistry of RUBICON in human kidney tissue with RUBICON antibody at 5 µg/ml.

Immunofluorescence of RUBICON in human kidney tissue with RUBICON antibody at 20 µg/ml.

Quick Database Links

Gene Symbol

KIAA0226

UniProt

Q92622

RefSeq:XP_005269431