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| Catalog Number | orb1239104 |
|---|---|
| Category | Antibodies |
| Description | Rubicon Antibody |
| Target | KIAA0226 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | 1 mg/mL |
| Buffer/Preservatives | Rubicon antibody is supplied in PBS containing 0.02% sodium azide. |
| Purification | Rubicon antibody is affinity chromatography purified via peptide column. |
| Immunogen | Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon.The immunogen is located within amino acids 240 - 290 of Rubicon. |
| UniProt ID | Q92622 |
| MW | Predicted: 90, 101, 109 kDa Observed: 91, 99 kDa |
| Tested applications | ELISA, IF, IHC-P, WB |
| Application notes | Rubicon body can be used for detection of Rubicon by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Run domain Beclin-1 interacting and cysteine-rich Read more... |
| Research Area | Cell Biology |
| Note | For research use only |
| NCBI | XP_005269431 |

Western blot analysis of Rubicon in 293 cell lysate with Rubicon antibody at 1 µg/ml.

Immunohistochemistry of RUBICON in human kidney tissue with RUBICON antibody at 5 µg/ml.

Immunofluorescence of RUBICON in human kidney tissue with RUBICON antibody at 20 µg/ml.
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