BARON Antibody (N-term)

SKU: orb1933260

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Immunology & Inflammation, Metabolism Research

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: IF - 1:100, WB - 1:2000, IHC-P - 1:50-100, FC - 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB18304
• Immunogen: This BARON antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-271 amino acids from the N-terminal region of human BARON. Antigen Region: 241-271 aa.
• Target: RUBCN (HGNC:28991)
• Molecular Weight: 108622 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Run domain Beclin-1 interacting and cysteine-rich containing protein, Rubicon, Beclin-1 associated RUN domain containing protein, Baron, KIAA0226

Flow cytometric analysis of MDA-231 cells using BARON Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Fluorescent image of U251 cells stained with BARON (N-term) antibody. U251 cells were treated with Chloroquine (50 μM, 16 h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with BARON (N-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). BARON immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Formalin-fixed and paraffin-embedded human lymph with BARON Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-BARON Antibody (N-term) at 1:2000 dilution. Lane 1: K562 whole cell lysate. Lane 2: 293 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 109 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

RUBCN (HGNC:28991)

UniProt

Q92622

RefSeq (Protein):NP_001139114.1, NP_055502.1