BARON Antibody (Center)

SKU: orb1933259

This product can be custom made

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Immunology & Inflammation, Metabolism Research

Images & Validation

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Item 1 of 3

Tested Applications	FC, IHC-P, WB
Dilution Range	WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50
Reactivity	Human
Predicted Reactivity	Mouse

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	BTD30304
Immunogen	This BARON antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 656-684 amino acids from the Central region of human BARON. Antigen Region: 656-684 aa.
Target	RUBCN (HGNC:28991)
Molecular Weight	108622 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Run domain Beclin-1 interacting and cysteine-rich containing protein, Rubicon, Beclin-1 associated RUN domain containing protein, Baron, KIAA0226

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

BARON Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human kidney reacted with BARON Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-BARON Antibody (Center) at 1:1000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: Raji whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 109 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.