RPL27A Antibody

• Catalog Number: orb1270488
• Category: Antibodies
• Description: RPL27A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Monkey, Mouse, Rat
• Reactivity: Hamster, Human
• Isotype: Rabbit Ig
• Immunogen: This RPL27A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-138 amino acids from the C-terminal region of human RPL27A.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 17 kDa
• Target: RPL27A
• UniProt ID: P46776
• NCBI: P46776
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: 60S ribosomal protein L27a, RPL27A
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis in K562 cell line lysates (35 ug/lane).

Western blot analysis in CHO cell line lysates (35 ug/lane).

RPL27A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.