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Catalog Number | orb766236 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Ribosomal Protein L27A |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Concentration | 1 mg/ml |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Immunogen | The antiserum was produced against synthesized peptide derived from human RPL27A. AA range:99-148 |
MW | 20 |
Tested applications | ELISA, IF, IHC-P, WB |
Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Anti-RPL27A antibody, anti-60S ribosomal protein L Read more... |
Note | For research use only |
Western Blot analysis of various cells using Ribosomal Protein L27A Polyclonal Antibody diluted at 1:2000.
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunofluorescence analysis of A549 cells, using RPL27A Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from COLO cells, using RPL27A Antibody. The lane on the right is blocked with the synthesized peptide.
FC, IHC-P, WB | |
Mouse, Rabbit, Rat | |
Hamster, Human | |
Rabbit | |
Polyclonal | |
Unconjugated |