RFX1 Antibody

SKU: orb1238773

Description

RFX1 Antibody

Research Area

Cell Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  RFX1 antibody can be used for detection of RFX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: RFX1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RFX1.The immunogen is located within the last 50 amino acids of RFX1.
• Target: RFX1
• Molecular Weight: Predicted: 108 kDa Observed: 125 kDa
• Purification: RFX1 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: RFX1 antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RFX, MHC class II regulatory factor RFX, MHC class II regulatory factor RFX1, enhancer factor C, EFC, trans-acting regulatory factor 1, transcription factor RFX1

Western blot analysis of RFX1 in HeLa cell lysate with RFX1 antibody at (A) 1 and (B) 2 µg/ml.

Immunocytochemistry of RFX1 in HeLa cells with RFX1 at 5 µg/mL.

Immunofluorescence of RFX1 in Hela cells with RFX1 antibody at 20 µg/mL. Green: RFX1 Antibody (orb1238773) Blue: DAPI staining.

Immunocytochemistry of RFX1 in Hela cells with RFX1 antibody at 5 µg/mL.

Quick Database Links

Gene Symbol

RFX1

UniProt

P22670

RefSeq (Protein):NP_002909