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Item 1 of 3
RFX1 Antibody (C-term)
Catalog Number: orb1934615
| Catalog Number | orb1934615 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB30347 |
| Tested applications | IF, WB |
| Predicted Reactivity | Mouse |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:2000 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 104758 Da |
| Target | This RFX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 896-925 amino acids from the C-terminal region of human RFX1. |
| UniProt ID | P22670 |
| NCBI | NP_002909.4 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | MHC class II regulatory factor RFX1, Enhancer fact Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
RFX1 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35 ug/lane). This demonstrates the RFX1 antibody detected the RFX1 protein (arrow).
All lanes: Anti-RFX1 Antibody (C-term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: K562 whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: PC-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 105 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Fluorescent image of U251 cell stained with RFX1 Antibody (C-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RFX1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). RFX1 immunoreactivity is localized to Nucleus significantly.
