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Rabbit anti-RFX1 Antibody
Catalog Number: orb1525560
| Catalog Number | orb1525560 |
|---|---|
| Category | Antibodies |
| Description | RFX1 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IHC, IP, WB |
| Reactivity | Human, Mouse |
| Immunogen | Between 929 and C-terminus |
| Antibody Type | Primary Antibody |
| Concentration | 200 µg/ml |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | RFX1 |
| UniProt ID | P22670 |
| NCBI | NP_002909.4 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Alternative names | MHC class II regulatory factor RFX; transcription Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of mouse RFX1 by western blot. Samples: Whole cell lysate (25 µg) from 3T3-L1, CT26, BW5147.3, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody (orb1525560) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human RFX1 by western blot. Samples: Whole cell lysate (5 µg) from HeLa, MCF-7, HEK293T, K-562, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody (orb1525560) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Detection of human RFX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFX1 antibody (orb1525560) used for IP at 6 µg per reaction. RFX1 was also immunoprecipitated by a previous lot of this antibody (orb1525560) and a second antibody against a different epitope of RFX1.
Detection of human RFX1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RFX1 (Cat. No. orb1525560) used at a dilution of 1: 1, 000 (0.2µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Detection of mouse RFX1 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RFX1 (Cat. No. orb1525560) used at a dilution of 1: 1, 000 (0.2µg/ml). Detection: DAB
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