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Catalog Number | orb1274250 |
---|---|
Category | Antibodies |
Description | Recombinant TNF-alpha Antibody [RMTNFA-1] |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | RMTNFA-1 |
Tested applications | IF, IHC-P |
Reactivity | Human |
Isotype | IgG, k |
Immunogen | Recombinant human protein was used as the immunogen for the TNF-alpha antibody. |
Antibody Type | Primary Antibody |
Concentration | 0.2 mg/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | TNF |
UniProt ID | P01375 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Tumor necrosis factor, Cachectin, TNF-alpha, Tumor Read more... |
Note | For research use only |
Application notes | Immunofluorescence: 5-10 ug/mlImmunohistochemistry (FFPE): 5-10 ug/ml for 30 min at RT (1)Optimal dilution of the TNF-alpha antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed, paraffin-embedded human pancreas stained with TNF alpha antibody.
Formalin-fixed, paraffin-embedded human histiocytoma stained with TNF alpha antibody.