Recombinant SOX2 (Embryonic Stem Cell Marker) Antibody

• Catalog Number: orb607713
• Category: Antibodies
• Description: Mouse monoclonal antibody to SOX2 (Embryonic Stem Cell Marker)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: rSOX2/1792
• Tested applications: ELISA, IHC
• Reactivity: Human, Mouse
• Isotype: IgG1
• Immunogen: Recombinant fragment (within aa176-305) of human SOX2 protein (exact sequence is proprietary)
• Antibody Type: Recombinant Antibody
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 34kDa
• Target: SOX2
• Entrez: 6657
• UniProt ID: P48431
• Expression System: HEK293 cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: ANOP3; Delta EF2a; MCOPS3 (Microphthalmia Syndromi
• Note: For research use only
• Application notes: Positive Control: MCF-7 cells. Lung or Cervical carcinoma
• Expiration Date: 12 months from date of receipt.