Recombinant SOX2 Antibody

SKU: orb2643396

Description

SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Reportedly, SOX2 is associated with aggressive phenotypes of breast, head and neck, gastric, colorectal, bladder, and small cell lung cancers. However, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and has been shown to be an independent favorable prognostic marker.

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution Range: ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant SOX2 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: SOX2/3811R
• Immunogen: A recombinant human partial protein (amino acids 176-305) was used as the immunogen for the recombinant SOX2 antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human cervical carcinoma with recombinant SOX2 antibody (clone SOX2/3811R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human cervical carcinoma with recombinant SOX2 antibody (clone SOX2/3811R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Quick Database Links

UniProt

P48431