Recombinant CD40 / TNFRSF5 / CD40L-Receptor Antibody

• Catalog Number: orb751435
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD40
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: C40/4826R
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fragment of human CD40 protein (around aa 29-107) (exact sequence is proprietary)
• Antibody Type: Recombinant Antibody
• Concentration: 200 μg/ml
• Dilution range: ELISA (For coating, order antibody without BSA); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 43kDa
• Target: CD40
• Entrez: 958
• UniProt ID: P25942
• Expression System: HEK293 cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: Anti-B-cell surface antigen CD40 antibody, anti-Bp
• Note: For research use only
• Application notes: Positive Control: U2OS or L-428 cells. Human tonsil, spleen or thymus.
• Expiration Date: 12 months from date of receipt.