TRAP Antibody (N-term)

• Catalog Number: orb1930000
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB19150
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Porcine
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 29274 Da
• Target: This TRAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-62 amino acids from the N-terminal region of human TRAP.
• UniProt ID: P29965
• NCBI: NP_000065.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-rela
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

TRAP antibody (N-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of TRAP Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

TRAP Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TRAP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of TRAP antibody (N-term) in NCI-H460 cell line lysates (35 ug/lane). TRAP (arrow) was detected using the purified Pab.

All lanes: Anti-TRAP Antibody (N-term) at 1:1000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: THP-1 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 29 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.