CD40/TNFRSF5 Rabbit Polyclonal Antibody

• Catalog Number: orb101846
• Category: Antibodies
• Description: CD40/TNFRSF5 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine
• Reactivity: Human, Mouse, Rabbit, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD40/TNFRSF5. (201-277/277aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 44-50 kDa
• Target: Cd40
• UniProt ID: P25942
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Tumor necrosis factor receptor superfamily, member
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human tonsil tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD40) Polyclonal Antibody, Unconjugated (orb101846) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-CD40 (orb101846) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kD, Observed band size: 52 kD.

Sample: Jurkat (Human) Cell Lysate at 30 ug, Raw264.7 (Mouse) Cell Lysate at 30 ug, Primary: Anti-CD40 (orb101846) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30 kD, Observed band size: 52 kD.

Sample: Lane 1: Spleen (Mouse) Lysate at 40 ug, Lane 2: Lung (Mouse) Lysate at 40 ug, Lane 3: Lymph node (Rat) Lysate at 40 ug, Primary: Anti-CD40/TNFRSF5 (orb101846) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44-50 kD, Observed band size: 50 kD.