Rat IgG F(ab')2 Antibody Peroxidase Conjugated

• Catalog Number: orb346701
• Category: Antibodies
• Description: Rat IgG F(ab')2 antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Rat
• Isotype: IgG
• Immunogen: Rat IgG F(ab')2 fragment
• Antibody Type: Secondary Antibody
• Concentration: 10.0 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against Rat IgG F(c).
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: None
• Alternative names: rabbit anti-Rat IgG F(ab')2 Peroxidase Conjugated
• Note: For research use only
• Application notes: Anti-Rat IgG F(ab’)2 antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.