F(ab')2 Rat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

SKU: orb348373

Description

F(ab')2 Rat IgG (H&L) antibody (Peroxidase)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:5,000 - 1:20,000, IHC: 1:500 - 1:2,000, WB: 1:500 - 1:2,000
• Reactivity: Rat
• Application Notes:
  Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Do not use sodium azide as a preservative. Azide will substantially inhibit the enzyme activity of horseradish peroxidase.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Donkey
• Clonality: Polyclonal
• Isotype: IgG F(ab')2
• Immunogen: Rat IgG whole molecule
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep serum proteins.

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Alternative Names

Donkey F(ab')2 Anti-Rat IgG Peroxidase Conjugated Antibody, Donkey F(ab')2 Anti-Rat IgG Antibody HRP Conjugation