Rabbit ZEB1 Antibody

SKU: orb1527548

Description

ZEB1 Antibody

Research Area

Cancer Biology, Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1074 and C-terminus
• Target: ZEB1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AREB6; BZP; delta-crystallin enhancer binding factor 1; DELTAEF1; FECD6; negative regulator of IL2; NIL2A; NIL-2-A zinc finger protein; posterior polymorphous corneal dystrophy 3; PPCD3; TCF8; TCF-8; Transcription factor 8; transcription factor 8 (represses interleukin 2 expression); ZFHEP; ZFHX1A; zinc finger E-box-binding homeobox 1; zinc finger homeodomain enhancer-binding protein

Detection of human and mouse ZEB1 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HeLa, A-549, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZEB1 antibody orb1527548 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human ZEB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZEB1 antibody orb1527548 used for IP at 6 µg per reaction.

Detection of mouse ZEB1 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-ZEB1 (Cat. No. orb1527548) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Detection of human ZEB1 by immunohistochemistry.Sample: FFPE section of humanglioblastoma. Antibody: Affinity purified rabbit anti-ZEB1 (Cat. No. orb1527548) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

ZEB1

UniProt

P37275

RefSeq (Protein):NP_110378.3