ZEB1 Rabbit Polyclonal Antibody

• Catalog Number: orb573707
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ZEB1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rabbit, Rat, Zebrafish
• Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ZEB1
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 124kDa
• Target: ZEB1
• UniProt ID: P37275
• Protein Sequence: Synthetic peptide located within the following region: NPRRNNVTNYNTVVETNSDSDDEDKLHIVEEESVTDAADCEGVPEDDLPT
• NCBI: NP_110378
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: BZP, TCF8, AREB6, FECD6, NIL2A, PPCD3, ZFHEP, ZFHX
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample Tissue: Human 786-0 Whole Cell, Antibody dilution: 1 ug/ml.

Sample Tissue: Human 786-0 Whole Cell, Antibody dilution: 1 ug/ml.

Lanes: 1. 45 ug capan1 cell lysate, 2. 45 ug HPAF cell lysate, Primary Antibody dilution: 1:1000, Secondary Antibody: Anti-Rabbit HRP, Secondary Antibody dilution: 1:5000, Gene Name: ZEB1.

Sample Type: Human Capan1 cells (Pancreatic cancer cell line), Primary Antibody dilution: 1:300, Secondary Antibody: Anti-rabbit-AlexaFluor-488, Secondary Antibody dilution: 1:200, Color/Signal Descriptions: ZEB: Green DAPI: Blue, Gene Name: ZEB1.

WB Suggested Anti-ZEB1 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:62500, Positive Control: 293T cell lysate.