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Item 1 of 4
Rabbit anti-PHGDH Antibody
Catalog Number: orb1522439
| Catalog Number | orb1522439 |
|---|---|
| Category | Antibodies |
| Description | PHGDH Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IHC, IP, WB |
| Reactivity | Human, Mouse |
| Immunogen | Between 1 and 50 |
| Antibody Type | Primary Antibody |
| Concentration | 1000 µg/ml |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | PHGDH |
| UniProt ID | O43175 |
| NCBI | NP_006614.2 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Alternative names | epididymis secretory protein Li 113; PGDH; PGD; PG Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human PHGDH by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHGDH antibody (orb1522439) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of mouse PHGDH by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHGDH antibody (orb1522439) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human PHGDH by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PHGDH antibody (orb1522439) used for IP at 6 µg per reaction. PHGDH was also immunoprecipitated by a previous lot of this antibody (orb1522439) and a second antibody against a different epitope of PHGDH.
Detection of mouse PHGDH by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-PHGDH (Cat. No. orb1522439) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
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