PHGDH Rabbit Polyclonal Antibody

SKU: orb582487

Description

Rabbit polyclonal antibody to PHGDH

Images & Validation

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Item 1 of 4

Tested Applications	WB
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat, Zebrafish

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the middle region of human PHGDH
Target	PHGDH
Molecular Weight	57 kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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NLS, PDG, PGD, NLS1, PGAD, PGDH, SERA, 3PGDH, 3-PGDH, PHGDHD, HEL-S-113

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment.

Rabbit Anti-PHGDH antibody, Formalin Fixed Paraffin Embedded Tissue: Human Pancreas, Primary antibody Concentration: 1:100, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20x, Exposure Time: 0.5-2.0 sec.

Rabbit Anti-PHGDH antibody, Formalin Fixed Paraffin Embedded Tissue: Human Prostate, Primary antibody Concentration: 1:100, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20x, Exposure Time: 0.5-2.0 sec.

WB Suggested Anti-PHGDH Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:62500, Positive Control: 293T cell lysate. PHGDH is supported by BioGPS gene expression data to be expressed in HEK293T.

Quick Database Links

Gene Symbol

PHGDH

UniProt

O43175

RefSeq (Protein):NP_006614

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_006614

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Protocol Information

Western Blot (IB, immunoblot)

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WB (1)

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Zebrafish (1)

Maria Marchese 1, Sara Bernardi 2, Rachele Vivarelli 2, Stefano Doccini 2, Lorenzo Santucci 2, Asahi Ogi 2, Rosario Licitra 3, Jingjing Zang 4, Rabah Soliymani 5, Serena Mero 2, Stephan Cf Neuhauss 4, Lea Ciarmoli 6, Giovanni Signore 6 7, Maciej M Lalowski 5 8, Filippo M Santorelli 9 CLN5 deficiency impairs glucose uptake and uncovers PHGDH as a potential biomarker in Batten disease Mol Psychiatry ., (2025)

PubMed: 40346285

Applications

Reactivity

Zebrafish