PHGDH Antibody (Center)

• Catalog Number: orb1931321
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB20883
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 56651 Da
• Target: This PHGDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249-277 amino acids from the Central region of human PHGDH.
• UniProt ID: O43175
• NCBI: NP_006614.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: D-3-phosphoglycerate dehydrogenase, 3-PGDH, PHGDH,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

PHGDH Antibody (Center) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

PHGDH Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PHGDH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

PHGDH Antibody (Center) western blot analysis in Ramos cell line lysates (15 ug/lane). This demonstrates the PHGDH antibody detected the PHGDH protein (arrow).