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Catalog Number | orb1529956 |
---|---|
Category | Antibodies |
Description | NONO Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human |
Immunogen | Between 1 and 50 |
Antibody Type | Primary Antibody |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | NONO |
UniProt ID | Q15233 |
NCBI | NP_031389.3 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | PPP1R114; protein phosphatase 1, regulatory subuni Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human NONO by western blot.Samples: Whole cell lysate from HEK293T, HeLa, Jurkat, MCF-7, and Hep-G2 cells. Antibody: Affinity purified rabbit anti-NONO antibody at 0.04 µg/ml.
Detection of human NONO by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- NONO used at a dilution of 1:100 (2µg/ml).
Detection of human NONO by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-NONO antibody.
IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |