Rabbit NONO Antibody

SKU: orb1529950

Description

NONO Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 350 and 400
• Target: NONO
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

54 kDa nuclear RNA- and DNA-binding protein; 55 kDa nuclear protein; DNA-binding p52/p100 complex, 52 kDa subunit; MRXS34; NMT55; NonO protein; non-POU domain-containing octamer (ATGCAAAT) binding protein; non-POU domain-containing octamer-binding protein; NRB54; P54; p54(nrb); P54NRB; PPP1R114; protein phosphatase 1, regulatory subunit 114

Detection of human NONO by western blot.Samples: Whole cell lysate from HEK293T, HeLa, Jurkat, MCF-7, and Hep-G2 cells. Antibody: Affinity purified rabbit anti-NONO antibody at 0.04 µg/ml.

Detection of mouse and rat NONO by western blot.Samples: Whole cell lysate from NIH 3T3, TCMK-1, 4T1, CT26, and C6 cells.Antibody: Affinity purified rabbit anti-NONO antibody at 0.04 µg/ml.

Detection of human NONO by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NONO used at a dilution of 1:1, 000 (0.2 µg/ml).

Detection of mouse NONO by immunohistochemistry. Sample: FFPE section of mouse intestine. Antibody: Affinity purified rabbit anti-NONO used at a dilution of 1:200 (1 µg/ml).

Detection of human NONO by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-NONO antibody.

Quick Database Links

Gene Symbol

NONO

UniProt

Q15233

RefSeq (Protein):NP_031389.3