Rabbit NELFE Antibody

SKU: orb1527566

Description

NELFE Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:1,000 - 1:5,000; IP - 2 - 5 µg/mg lysate
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 75 and 125
• Target: NELFE
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

D6S45; major histocompatibility complex gene RD; negative elongation factor E; negative elongation factor polypeptide E; NELF-E; nuclear protein; RD; RD RNA-binding protein; RDBP; RDP; RNA-binding protein RD

Detection of human NELFE by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NELFE antibody orb1527566 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. NELFE was also immunoprecipitated by a previous lot of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Quick Database Links

Gene Symbol

NELFE

UniProt

P18615

RefSeq (Protein):NP_002895.3