Anti-NELFE Antibody

• Catalog Number: orb1743879
• Category: Antibodies
• Description: Anti-NELFE Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human
• Immunogen: E.coli-derived human NELFE recombinant protein (Position: N262-D378).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 43 kDa
• UniProt ID: P18615
• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of 293T cells using anti-NELFE antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of NELFE using anti-NELFE antibody.Lane 1:human 293T cell;2:human HeLa cell;3:human U20S cell.

IF analysis of NELFE using anti-NELFE antibody and anti-Beta Tubulin antibody.NELFE was detected in immunocytochemical section of A549 cell.

IHC analysis of NELFE using anti-NELFE antibody. NELFE was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue.

IHC analysis of NELFE using anti-NELFE antibody. NELFE was detected in a paraffin-embedded section of human ovarian cancer tissue.