Rabbit anti-NELFE Antibody

• Catalog Number: orb1527564
• Category: Antibodies
• Description: NELFE Antibody
• Target: NELFE
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 330 and C-terminus
• UniProt ID: P18615
• Tested applications: IHC, IP
• Dilution range: WB - Not recommended. orb1527564 has not performed satisfactorily when used for WB of NELFE in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of NELFE.; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: negative elongation factor polypeptide E; RNA-bind
• Note: For research use only
• NCBI: NP_002895.3
• Expiration Date: 12 months from date of receipt.

Detection of human NELFE by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NELFE antibody orb1527564 used for IP at 3 µg/mg lysate.

Detection of human NELFE by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NELFE (Cat. No. orb1527564) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB