Rabbit IRF8 Antibody

SKU: orb1523734

Description

IRF8 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate
• Reactivity: Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 374 and C-terminus
• Target: IRF8
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AI893568; ICSBP; Icsbp1; interferon consensus sequence binding protein 1; interferon consensus sequence-binding protein; interferon regulatory factor 8; IRF-8; Myls

Detection of mouse IRF8 by western blot. Samples: Whole cell lysate (50 µg) from L1210cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IRF8 antibody orb1523734 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of mouse IRF8 by western blot. Samples: Whole cell lysate (15 and 50 µg) from RAW 274.7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IRF8 antibody orb1523734 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human IRF8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RAW 274.7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IRF8 antibody orb1523734 used for IP at 3 µg per reaction.

Quick Database Links

Gene Symbol

IRF8

UniProt

P23611

RefSeq (Protein):NP_032346.1