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Rabbit iASPP Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 75 and 125 |
| Target | iASPP |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Detection of human and mouse iASPP by western blot. Samples: Whole cell lysate from HEK293T (50, 15, 5 µg) , HeLa (50, 15 µg) , and mouse NIH 3T3 (50 µg) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-iASPP antibody orb1527881 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human iASPP by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-iASPP antibody orb1527881 used for IP at 6 µg per reaction.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_006654.2 |
|---|
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Protocol Information
Rabbit iASPP Antibody (orb1527881)
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