PPP1R13L Antibody (N-Term)

• Catalog Number: orb1926291
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This PPP1R13L antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 134-166 amino acids from human PPP1R13L.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q8WUF5
• MW: 89091 Da
• Tested applications: IF, WB
• Dilution range: IF: 1:25, WB: 1:2000
• Clone Number: RB53806
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: RelA-associated inhibitor, Inhibitor of ASPP prote
• Note: For research use only

All lanes: Anti-PPP1R13L Antibody (N-Term) at 1:2000 dilution. Lane 1: MCF-7 whole cell lysates. Lane 2: Hela whole cell lysates. Lane 3: NCI-H1299 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 89 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma cell line) cells labeling PPP1R13L at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).