Rabbit anti-DCK Antibody

• Catalog Number: orb1523314
• Category: Antibodies
• Description: DCK Antibody
• Target: DCK
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 210 and C-terminus
• UniProt ID: P27707
• Tested applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: dCK; deoxynucleoside kinase; deoxycytidine kinase
• Note: For research use only
• NCBI: NP_000779.1

Detection of human and mouse DCK by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-DCK antibody orb1523314 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human DCK by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DCK antibody orb1523314 used for IP at 6 µg per reaction.

Detection of human DCK by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DCK (Cat. No. orb1523314) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of mouse DCK by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-DCK (Cat. No. orb1523314) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB